Faculty

Julie Biteen

Assistant Professor of Chemistry

A.B. Chemistry, Princeton University, 2001
M.S. Applied Physics, Caltech 2003
Ph.D. Chemistry, Caltech, 2006
Postdoctoral Fellow, Stanford University, 2006-2009

Research Focus: Single-Molecule Fluorescence, Live-Cell Biomedical Imaging, Plasmonics, Nanophotonics

Phone: 734.647.1135
E-mail: jsbiteen@umich.edu


High-resolution, high-sensitivity biomedical imaging based on single-molecule fluorescence and nanophotonics
By beating the diffraction limit that restricts traditional light microscopy, single-molecule fluorescence imaging is a flexible, noninvasive way to sensitively probe position and dynamics.  Our lab has pioneered super-resolution imaging methods for applications to central processes that remain contested by microbiologists. Based on the methods that we have developed for single-molecule imaging in live bacteria cells, we have uncovered the organization of structural proteins, we have revealed the mechanism of membrane-bound transcription regulation in the pathogenic Vibrio cholerae, we have demonstrated the dynamic interactions involved in starch degradation in the human gut symbiont Bacteroides thetaiotaomicron, and we have uncovered an intimate and dynamic coupling between DNA mismatch recognition and DNA replication in a highly conserved repair pathway. 

Moreover, we have examined the fundamentals of plasmon-enhanced fluorescence with a combination of single-molecule imaging experiments and electromagnetic simulations, and we are now applying this understanding to biocompatible enhancement of fluorescent protein emission, extending the implications of metal-enhanced fluorescence to increased signal and resolution in live-cell bio-imaging, and creating a flexible technology for high-resolution, real-time imaging.

Biteen Research Group

 

Awards

2013 NSF CAREER Award
2011
Picoquant Young Investigator Award
2009-2013
Burroughs Wellcome Fund Career Award at the Scientific Interface
2009
University of Michigan Biological Sciences Scholar
2004 Society of Chemical Industry Perkin Medal Scholarship
2004 PEO Scholar Award

 

Representative Publications

  1. B.L. Haas, J.S. Mason, V.J. DiRita and JSB, “Single-molecule tracking in live Vibrio choleraereveals that ToxR recruits the membrane-bound virulence regulator TcpP to the toxTpromoter,” Molecular Microbiology, in press (2014). DOIhttp://dx.doi.org/10.1111/mmi.12834.

  2. T. Hu, B.P. Isaacoff, Y. Zhou, J. Zhu, X. Li, Z. Wang, S. Liu, JSB* and N.A. Kotov*, “Self-Organization of Plasmonic and Excitonic Nanoparticles into Resonant Chiral Supraparticle Assemblies,” Nano Letters, 14 6799-6810 (2014).

  3. K.S. Karunatilaka, E.A. Cameron, E.C. Martens, N.M. Koropatkin and JSB, “Super-Resolution Imaging Captures Carbohydrate Utilization Dynamics in Human Gut Symbionts,” mBio, 5e02172-14 (2014).

  4. J.E. Donehue, E. Wertz, C.N. Talicska and JSB, “Plasmon-Enhanced Brightness and Photostability from Single Fluorescent Proteins Coupled to Gold Nanorods,” Journal of Physical Chemistry C, 118 15027-15035 (2014).

  5. M.-C. Cheng, A. T. Leske, T. Matsuoka, B. C. Kim, J.-S. Lee, M. A. Burns, S. Takayama and JSB, “Super-Resolution Imaging of PDMS Nanochannels by Single-Molecule Micelle-Assisted Blink Microscopy,” Journal of Physical Chemistry B, 117 4406-4411 (2013).

  6. Y. Liao, S.K. Yang, K. Koh, A.J. Matzger and JSB, “Heterogeneous Single-Molecule Diffusion in One- Two- and Three-Dimensional Microporous Coordination Polymers: Directional, Trapped and Immobile Guests,” Nano Letters, 12 3080 (2012).

 

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